I am doing Kneaddata and humann3 for identifying the functional gene for shot-gun metagenome using commands:
kneaddata --input AST2R1.fastq --input AST2R1.fastq -db $/home/plankton/Kneadata_DIR --output /home/plankton/Metagenomics_AST_Thatha/CG_DN_935 --trimmomatic /home/plankton/anaconda3/share/trimmomatic-0.39-2 --thread 4
humann --input /home/plankton/Metagenomics_AST_Thatha/CG_DN_935/AST2.1/AST2R1_kneaddata.fastq --output /home/plankton/Metagenomics_AST_Thatha/CG_DN_935/AST2.1/AST2_humann --nucleotide-database /home/plankton/INSTALL_LOCATION --thread 8
In both the cases the system took long time and hanged.
Also, Firefox is crashing.
System config Ubuntu 20.04.3 LTS
df -h
Filesystem Size Used Avail Use% Mounted on udev
32G 0 32G 0% /dev tmpfs 6.3G 2.0M 6.3G 1%
/run /dev/sdb3 457G 283G 152G 66%
/ tmpfs 32G 112K 32G 1%
/dev/shm tmpfs 5.0M 4.0K 5.0M 1%
/run/lock tmpfs 32G 0 32G 0%
/sys/fs/cgroup
Crash report
ls -al /var/crash
total 53288
drwxrwsrwt 2 root whoopsie 4096 Jan 20 16:48 .
drwxr-xr-x 14 root root 4096 Aug 19 16:17 ..
-rw-r----- 1 plankton whoopsie 33984941 Jan 19 17:51 _usr_bin_gnome-shell.1000.crash
-rw-rw-r-- 1 plankton whoopsie 0 Jan 18 15:47 _usr_bin_gnome-shell.1000.upload
-rw------- 1 whoopsie whoopsie 37 Jan 18 15:47 _usr_bin_gnome-shell.1000.uploaded
-rw-r----- 1 plankton whoopsie 10929583 Jan 15 21:59 _usr_bin_nautilus.1000.crash
-rw-rw-r-- 1 plankton whoopsie 0 Jan 15 21:59 _usr_bin_nautilus.1000.upload
-rw------- 1 whoopsie whoopsie 37 Jan 15 21:59 _usr_bin_nautilus.1000.uploaded
-rw-r----- 1 plankton whoopsie 9621952 Jan 20 16:48 _usr_lib_xorg_Xorg.1000.crash
-rw-r--r-- 1 plankton whoopsie 0 Jan 20 16:48 _usr_lib_xorg_Xorg.1000.upload
-rw------- 1 whoopsie whoopsie 37 Jan 20 16:48 _usr_lib_xorg_Xorg.1000.uploaded
Please provide solution
Thank you